From: Directed evolution of a genetically encoded immobilized lipase for the efficient production of biodiesel from waste cooking oil
Construct
T50 (°C)
Residual activity after MeOH incubation (%)
Soluble PML
45.6 ± 0.38
25.3 ± 5.7
Soluble PMLVG
40.5 ± 0.25
22.0 ± 1.1
Cry3Aa–PML
51.7 ± 0.89
12.0 ± 2.3
Cry3Aa–PMLVG
51.2 ± 1.2
26.1 ± 1.2