Biotechnology for Biofuels and Bioproducts

Table 1 Masses of glucan, xylan, and lignin in solids for unpretreated switchgrass and following DSA and CELF pretreatments of switchgrass for varying presoaking times and particle sizes

From: CELF significantly reduces milling requirements and improves soaking effectiveness for maximum sugar recovery of Alamo switchgrass over dilute sulfuric acid pretreatment

Pretreatment	Presoaking time^a	Particle size^b	Glucan (g)	Xylan (g)	Lignin (g)
Unpretreated	–	–	38.8	26.8	21.7
DSA	0 h	< 1 mm	37.5	2.8	17.7
DSA	4 h	< 1 mm	36.3	2.7	17.8
DSA	18 h	< 1 mm	35.8	2.9	17.6
CELF	0 h	< 1 mm	37.4	2.7	4.6
CELF	4 h	< 1 mm	36.8	2.3	4.0
CELF	18 h	< 1 mm	37.0	1.7	3.8
DSA	18 h	Unmilled	36.0	2.7	18.6
DSA	18 h	< 2 mm	35.9	2.7	17.6
DSA	18 h	< 1 mm	35.8	2.9	17.6
CELF	18 h	Unmilled	37.2	1.6	4.1
CELF	18 h	< 2 mm	36.8	1.7	4.0
CELF	18 h	< 1 mm	37.0	1.7	3.8

^aDilute sulfuric acid (DSA) samples were presoaked in 0.5 wt% sulfuric acid, and Co-solvent Enhanced Lignocellulosic Fractionation (CELF) samples were presoaked in 0.5 wt% sulfuric acid at a 0.889:1 THF/water mass ratio, both at 4 °C
^bParticle size reduction achieved by knife milling

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ISSN: 2731-3654

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