Fig. 1

Geometric illustration of the eMOMA method. Red-colored area represents a solution space of flux vectors which can support maximum specific growth rate, while blue-colored area represents a solution space of flux vectors for nutrient-limited conditions. Upon nutrient starvation, the cells cannot retain a flux vector selected by pFBA and should adjust their fluxes from the red-colored area to the blue-colored area. eMOMA assumes that the cells try to minimize the metabolic adjustments in response to environmental perturbations (represented by black perpendicular dotted lines). Illustrations for a wild-type and b mutant situations are provided