Table 2 Top ten knockout targets for increasing lipid production predicted by eMOMA-based design method
Reaction abbreviation | Reaction description | EC number | Genes associated | Predicted yield improvement (%) |
|---|---|---|---|---|
MTHFC | Methenyltetrahydrofolate cyclohydrolase | EC 3.5.4.9 | (YALI0F30745g and YALI0E01056g) | 57.1 |
FTHFL | Formate-tetrahydrofolate ligase | EC 6.3.4.3 | (YALI0E01056g and YALI0F30745g) | 51.9 |
MTHFD | Methylenetetrahydrofolate dehydrogenase (NADP+) | EC 1.5.1.5 | (YALI0F30745g and YALI0E01056g) | 51.1 |
ALPHNH | Allophanate hydrolase | EC 3.5.1.54 | YALI0E07271g | 50.9 |
UREASE | Urea carboxylase | EC 6.3.4.6 | YALI0E07271g | 50.9 |
ARGN | Arginase | EC 3.5.3.1 | YALI0E07535g | 50.9 |
ERGSTt | Ergosterol reversible transport (extracellular) | n/a | YALI0F17996g | 48.0 |
GHMT2r | Glycine hydroxymethyltransferase | EC 2.1.2.1 | (YALI0D22484g and YALI0E16346g) | 15.5 |
IPPSm | 2-Isopropylmalate synthase (mitochondrial) | EC 2.3.3.13 | YALI0B07447g | 10.7 |
ICDHym | Isocitrate dehydrogenase (NADP+, mitochondrial) | EC 1.1.1.42 | YALI0F04095g | 9.3 |