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Fig. 4 | Biotechnology for Biofuels

Fig. 4

From: Secretomic analyses of Ruminiclostridium papyrosolvens reveal its enzymatic basis for lignocellulose degradation

Fig. 4

Comparison of expression and activity of CAZymes from four secretomes. a Functional and structural classification of the CAZymes released by R. papyrosolvens grown on glucose, cellobiose, cellulose and corn stover. b Distribution of abundance of cellulosomal CAZymes and free CAZymes in various conditions (*P < 0.05, **P < 0.01, t-test). c Proportion of major CAZyme families in total expressed CAZymes under four culture conditions. d Expression profiles of CAZymes under the selected carbon sources were clustered into four groups by hierarchical clustering analysis. The structural [cellulosomal component (CC) and non-cellulosomal enzyme (NC)] and functional [glycoside hydrolase (GH), glycosyltransferase (GT), carbohydrate esterase (CE), polysaccharide lyase (PL) and unknown function enzyme (UN)] characteristics of CAZymes were distinguished by different color blocks. e Comparative hydrolysis of cellulosic CMC and Avicel, xylan and corn stover by enzymes in the secretomes of R. papyrosolvens grown on glucose, cellobiose, cellulose and corn stover. The values shown are the means of three replicates, and the error bars indicate standard deviations from the mean values (*P < 0.05, **P < 0.01, t-test)

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