Application of an in situ CO2–bicarbonate system under nitrogen depletion to improve photosynthetic biomass and starch production and regulate amylose accumulation in a marine green microalga Tetraselmis subcordiformis

Table 3 Dissipated energy flux per excited cross section (DIo/CS₀), carotenoid/chlorophyll ratio (Car/Chl) and DIC (CO₂, HCO₃⁻ and CO₃²⁻) concentrations in the medium of T. subcordiformis cultures with different amounts of NaHCO₃ addition under nitrogen limitation (Day 8, mean ± SD, n = 3)

NaHCO₃ (g L⁻¹)	DIo/CS₀	Car/Chl	CO₂ (mmol kgSW⁻¹)			HCO₃⁻ (mmol kgSW⁻¹)			CO₃²⁻ (mmol kgSW⁻¹)
NaHCO₃ (g L⁻¹)	DIo/CS₀	Car/Chl	1 day	2 days	8 days	1 day	2 days	8 days	1 day	2 days	8 days
0	125 ± 22^A	0.301 ± 0.004^AB	0.03 ± 0.01	0.08 ± 0.03	0.11 ± 0.02	2.54 ± 0.07	4.84 ± 1.35	3.70 ± 0.03	0.17 ± 0.03	0.27 ± 0.04	0.11 ± 0.02
0.2	120 ± 14^A	0.302 ± 0.007^AB	0.03 ± 0.01	0.07 ± 0.01	0.07 ± 0.01	2.90 ± 0.49	6.60 ± 0.20	4.87 ± 0.61	0.27 ± 0.05	0.57 ± 0.05	0.29 ± 0.09
1	95 ± 24^A	0.311 ± 0.006^B	0.03 ± 0.00	0.04 ± 0.02	0.07 ± 0.01	6.73 ± 0.30	8.36 ± 3.57	10.18 ± 0.73	1.24 ± 0.06	1.38 ± 0.53	1.28 ± 0.15
5	352 ± 87^B	0.295 ± 0.005^A	0.03 ± 0.01	0.06 ± 0.01	0.06 ± 0.01	16.17 ± 2.30	24.74 ± 1.61	26.29 ± 2.86	8.36 ± 0.92	10.21 ± 1.35	10.76 ± 0.80

The different capital Latin alphabets (A and B) represented significant difference (p < 0.05) between the cultures

ISSN: 2731-3654