Table 1 n-Butanol production of different enzyme combinations
From: Metabolic engineering of Clostridium thermocellum for n-butanol production from cellulose
Combination number | n-Butanol pathway | n-Butanol (mg/L) | Ethanol (mg/L) | Acetate (mg/L) | Lactate (mg/L) | ||
|---|---|---|---|---|---|---|---|
BT01 | Thl-Hbd-Crt | Bcd-EtfAB | AdhE2 | 10 ± 2a | 3100 ± 100 | 2800 ± 100 | 1500 ± 200 |
BT02 | Bad-Bdh | 75 ± 6 | 2600 ± 100 | 2400 ± 100 | 1400 ± 200 | ||
BT03 | Bad-Bdh(Ca) | 15 ± 1 | 3200 ± 200 | 3000 ± 100 | 1500 ± 300 | ||
BT04 | Ter | AdhE2 | 13 ± 1 | 3100 ± 200 | 2900 ± 200 | 1200 ± 200 | |
BT05 | Bad-Bdh | 87 ± 8 | 2500 ± 200 | 2200 ± 100 | 1100 ± 100 | ||
BT06 | Bad-Bdh(Ca) | 20 ± 2 | 3100 ± 200 | 2700 ± 100 | 1200 ± 200 | ||
BT07 | PhaAB-PhaJ | Bcd-EtfAB | AdhE2 | 5 ± 1 | 2900 ± 100 | 3200 ± 200 | 1300 ± 200 |
BT08 | Bad-Bdh | 54 ± 3 | 2900 ± 200 | 3000 ± 100 | 1200 ± 100 | ||
BT09 | Bad-Bdh(Ca) | 10 ± 2 | 3100 ± 100 | 2800 ± 100 | 1200 ± 100 | ||
BT10 | Ter | AdhE2 | 6 ± 1 | 3100 ± 100 | 2900 ± 200 | 1300 ± 300 | |
BT11 | Bad-Bdh | 41 ± 5 | 2900 ± 200 | 2600 ± 100 | 1600 ± 200 | ||
BT12 | Bad-Bdh(Ca) | 8 ± 1 | 3100 ± 200 | 3000 ± 100 | 1500 ± 100 | ||