Fig. 2
From: A screening approach for assessing lytic polysaccharide monooxygenase activity in fungal strains
Saturation of Celluclast 1.5L with β-glucosidase and xylanases. Enzymatic blending was done for saturation of Celluclast 1.5L with different concentrations of β-glucosidase and xylanases. Minimum saturating concentrations of β-glucosidase enzymes suitable for 7 FPU of Celluclast 1.5L were defined based on depletion of cellobiose concentrations in the hydrolysates. After β-glucosidase saturation, xylanases were also blended to determine the minimum saturating xylanase concentration. 500 U of xylanase concentration was optimal to achieve saturation. The effect of enzyme supplementation was evaluated by recording % saccharification of pretreated rice straw at 24 h (white bars) and 48 h (gray bars) of hydrolysis using various enzymatic combinations. The error bars represent the standard deviation of three independent experiments. Symbol C represents Celluclast 1.5L (7 FPU), BGL represents commercial β-glucosidase enzyme (Novozymes 188), and Xyl represents commercial xylanase enzyme (Cellic® HTec3)