Fig. 5
Transcription levels of major cellulase-related genes in Δ76238 using glucose as the carbon source. Transcriptional levels of major cellulase-related genes cbh1 (a), cbh2 (b), egl1 (c), egl2 (d), and xyr1 (e) were evaluated via quantitative real-time PCR (qPCR). T. reesei strains were grown using glucose for 72, 96, or 120 h. The data are normalized to the expression of RUT-C30 over 72 h for each tested gene, with the sar gene used as an endogenous control in all samples. Values are the mean ± SD of the results from three independent experiments. Asterisks indicate significant differences (***p < 0.001, Student’s t test)