Fig. 3

Characterization of CRISPR-Cas9/Cas9n mediated genome editing. a Editing efficiency and CFU for sequence deletions using CRISPR/Cas9 or CRISPR/Cas9n. For deletion, the region of the amyE gene and flanking sequences were deleted. b Editing efficiency and CFU for gene insertions. A deleted sequence in the amyE region was restored. c Editing efficiency and CFU for large genomic deletions. A prophage (-like) regions was deleted. In these genetic modifications, 500 bp homologous-arms were used for recombination. All error bars represent the value of standard deviation which were calculated from three repeated experiments