Fig. 1
The amylolytic S. cerevisiae Y294 strains were evaluated at 30 °C under oxygen-limited conditions in 100 ml fermentation bottles containing 2 × SC–URA broth supplemented with 5 g l−1 glucose and 200 g l−1 raw corn starch as carbohydrate sources. The a ethanol and b glucose production was monitored overtime. The S. cerevisiae Y294[TemG_Opt-TemA] strain was cultivated in a 2-l bioreactor, c ethanol and residual glucose concentrations at 26 and 30 °C, and d the percentage estimated carbon conversion at 26 and 30 °C. Values represent the mean of three repeats and error bars represent the standard deviation