Fig. 2From: Adaptively evolved Escherichia coli for improved ability of formate utilization as a carbon source in sugar-free conditionsFormate utilization and biomass production at various stages of E. coli strain development with iOD of 0.7. The strains grew in formate M9 minimal medium (a–c) and formate M9 minimal medium supplemented with 1 g/L of glycine (d–f). Wild-type: Escherichia coli BL21; EM: FtfL overexpression in E. coli; EMK: FtfL, MtdA and Fch overexpression in E. coli; EMK00: gcvP knockout in the EMK strain; EMK01: GlyA overexpression in the EMK00 strain; EMK02: Sga, Hpr, and Gck overexpressions in the EMK01 strain; Black circles: Optical density at 600 nm (OD600); White circles: formate concentrationsBack to article page