Fig. 5

Functional confirmation of the mutations in EMK02A2. The tetrahydrofolate (THF) cycle pathway in E. coli (a). purN, encoding phosphoribosylglycinamide formyltransferase 1; purU, encoding formyltetrahydrofolate hydrolase; purT, encoding phosphoribosylglycinamide formyltransferase 2; metF, encoding 5,10-methylenetetrahydrofolate reductase; DHF, dihydrofolate; THF, tetrahydrofolate; 5-MTHF, 5-methyl-THF. The formate hydrogen lyase (FHL) system (b). FhlA is an activator of the FHL system, and FhlA is repressed by HycA and Fnr. Red letters: the deleted genes or proteins. Effects on growth and formate uptake rates by the deletions of one of the THF cycle genes or of the FHL system in EMK02 grown in EMK medium at iOD of 0.1 (c). Effects by deletions of two genes of the THF cycle and/or the THF system (d)