Fig. 6

FDCA production by AAO along time in the absence (closed circles) and presence of catalase (open circles) using HMF (a), DFF (b), and FFCA (c) as substrate. Dashed lines represent AAO residual activity along the time course of the reaction, enabling half-life (t_1/2) calculation. Reactions between 1.5 mM substrate and 1.5 µM AAO (and catalase 2–5 U/mL) were performed in 50 mM sodium phosphate, pH 6.0, at 28 °C