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Table 1 Kinetic parameters for the oxidation of p-methoxybenzyl alcohol and p-anisaldehyde by AAO in the presence of different H2O2 concentrations

From: Complete oxidation of hydroxymethylfurfural to furandicarboxylic acid by aryl-alcohol oxidase

p-Methoxybenzyl alcohol p-Anisaldehyde
[H2O2] (mM) kcat (s−1) Km (µM) kcat/Km (s−1mM−1) [H2O2] (mM) kcat (min−1)
0 108 ± 3 31.8 ± 3.2 3390 ± 360 0 7.3 ± 0.7
0.5 115 ± 3 44.3 ± 3.5 2600 ± 220 1.0 7.3 ± 0.2
1.0 104 ± 3 33.5 ± 3.4 3100 ± 330 1.5 6.5 ± 0.3
1.9 109 ± 4 35.6 ± 4.3 3060 ± 390 3.1 6.7 ± 0.6
7.7 100 ± 4 33.7 ± 4.5 2970 ± 420 9.3 6.9 ± 0.5
15.5 97 ± 3 31.0 ± 2.6 3120 ± 280 12.3 6.9 ± 0.1
  1. Activity was followed by measuring aldehyde formation (in p-methoxybenzyl alcohol oxidation) or acid formation (in p-anisaldehyde oxidation). Turnovers for aldehyde oxidation were calculated at saturating concentration of p-anisaldehyde (~ 5 mM) at 25 °C in air-saturated (0.256 mM O2) 50 mM sodium phosphate, pH 6.0