Table 3 Catalytic performance parameters for the production of FDCA from HMF by native AAO, and its F501H, F397Y, and I500M/F501 W variants, in the presence of catalase (10–17 U/mL) in 50 mM sodium phosphate, pH 6.0, at 28 °C, after 6 days of reaction
From: Complete oxidation of hydroxymethylfurfural to furandicarboxylic acid by aryl-alcohol oxidase
AAO variant | [HMF] (mM) | % conversion | [FDCA] mM | TTN |
|---|---|---|---|---|
WT | 1.5 | 62.4 | 0.9 | 1980 |
15 | 26.6 | 4.2 | 8440 | |
F501H | 1.5 | 97.9 | 1.6 | 3100 |
15 | 43.0 | 6.8 | 13,600 | |
F397Y | 1.5 | 75.7 | 1.2 | 2400 |
15 | 45.0 | 7.1 | 14,300 | |
I500M/F501W | 1.5 | 52.8 | 0.8 | 1670 |
15 | 50.8 | 8.0 | 16,100 |