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Table 3 Catalytic performance parameters for the production of FDCA from HMF by native AAO, and its F501H, F397Y, and I500M/F501 W variants, in the presence of catalase (10–17 U/mL) in 50 mM sodium phosphate, pH 6.0, at 28 °C, after 6 days of reaction

From: Complete oxidation of hydroxymethylfurfural to furandicarboxylic acid by aryl-alcohol oxidase

AAO variant [HMF] (mM) % conversion [FDCA] mM TTN
WT 1.5 62.4 0.9 1980
15 26.6 4.2 8440
F501H 1.5 97.9 1.6 3100
15 43.0 6.8 13,600
F397Y 1.5 75.7 1.2 2400
15 45.0 7.1 14,300
I500M/F501W 1.5 52.8 0.8 1670
15 50.8 8.0 16,100