Fig. 1
From: Discovery and implementation of a novel pathway for n-butanol production via 2-oxoglutarate
Schematic representation of the successive stages of the in silico analysis. The pathways to produce butanol in E. coli were generated using the algorithms described in [13], were filtered and ranked, resulting in a set of the 24 most promising alternatives. The reactions constituting the final set of pathways are shown with indication of the respective KEGG ID. These pathways are constituted by the following enzymatic activities: 2-oxoglutarate reductase (R08198 and R03534); glutaconate-CoA transferase (R04000); 2-hydroxyglutaryl-CoA dehydratase (R03937); glutaconyl-CoA decarboxylase (R03028); butyryl-CoA dehydrogenase (R01171; R01175 and R09738); aldehyde dehydrogenase (R01172 and R01173) and alcohol dehydrogenase (R03544 and R03545). GSMM genome-scale metabolic model, ButOH butanol