Fig. 4

Genome-wide d_N/d_S distribution across homeologs in 2T2.1 shows the same distribution as orthologues across species, indicating that the source of this duplication was likely a hybridization event (allopolyploidization). Histograms (green) show d_N/d_S distribution across duplicated single-copy genes from Coniochaeta sp. 2T2.1 (top left) and single-copy orthologues across: Coniochaeta sp. PMI 546 and C. lignaria CBS111746 (top right), Coniochaeta sp. PMI546 and C. lignaria NRRL30616 (middle right), and Lecythophora sp. AK0013 and Coniochaeta sp. PMI546 (bottom right). Quantile–Quantile plots were then generated to compare d_N/d_S distribution in 2T2.1 homeologs with orthologues between species, revealing that distributions are highly correlated (R² ≥ 0.945)