Fig. 3

Reconstruction of Clostridium sp. AWRP central metabolism based on the genomic information. Locus numbers of the corresponding genes are shown in green on each reaction without the locus prefix. A lumped reaction or pathway is shown in a dashed arrow for simplicity; the gray box indicates l-methionine auxotrophy of strain AWRP. It was assumed that three to four protons are required to phosphorylate one ATP according to a previous study on Clostridium paradoxum [54], and that two protons are translocated per ferredoxin oxidized [8, 55]. The electron donor of the methylenetetrahydrofolate reductase is indicated with question marks since it is still unclear in clostridial species [17]