Fig. 1

Liquid fraction (LF) supports the degradation of chitin by SmLPMO10A. Reactions were made in 50 mM sodium acetate (pH 5.0) at 25 °C. The concentration of CNWs was 1.0 g L⁻¹ and that of SmLPMO10A was 0.05 µM (except in one series where it was 0.1 µM, as indicated in the Figure). Before use in the reactions, the LF was pre-incubated at 25 °C for overnight. The amount of LF was 5%, 10%, or 15% of the total reaction volume. a Time curves of the formation of soluble products (expressed in N-acetylglucosamine equivalents, NAG_eq) upon incubation of CNWs with SmLPMO10A in the presence of LF. Solid lines show the best-fit of non-linear regression analysis according to Eq. 1. Control experiments show the results obtained (i) with 10% LF but in the presence of 1-µM horseradish peroxidase (note that no extra HRP substrate was added, since the LF contains HRP substrates), (ii) with 10% LF but in the absence of SmLPMO10A, or (iii) in the absence of LF. Note that no reductant was added to these reaction mixtures. b, c Dependence of (b) n[H₂O₂]_t=0, and (c) \(nv_{{{\text{H}}_{2} {\text{O}}_{2} }}\) on the concentration of LF in the LPMO reaction. The values of n[H₂O₂]_t=0 and \(nv_{{{\text{H}}_{2} {\text{O}}_{2} }}\) were found by analysis of the data in a using non-linear regression analysis according to Eq. 1 (opened black symbols) or linear regression analysis (only the data points measured between 10 and 120 min were included here) according Eq. 2 (open gray symbols). Filled black symbols represents the data obtained with 10% LF and double the amount (0.1 µM) of SmLPMO10A. Error bars represent S.D. and are from at least two independent measurements