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Table 2 Plasmids and strains used in this study

From: Single mutation at a highly conserved region of chloramphenicol acetyltransferase enables isobutyl acetate production directly from cellulose by Clostridium thermocellum at elevated temperatures






BacillusE. coli shuttle vector, CmR, pBC1 ori for Gram-positive strains, pBR322 ori for E. coli, source of CATSa

Bacillus Genetic Stock Center


pBR322 ori, AmpR, lacI, T7lac promoter



CATSa wild-type encoding gene between BamHI, SacI site, pETDuet-1 backbone, 6× His-tag at N-terminus

This study


F97W site-directed variant, pET_CATSa backbone

This study


CATSa wild-type gene under C. thermocellum PgapDH promoter, downstream of Clo1313_2927 for the transcription terminator, tdk operon under cbp promoter substituting with the native cat selection marker, pNW33N plasmid backbone

This study


CATSa F97W site-directed mutated from pHS0024

This study


 E. coli Top10

Host for molecular cloning, mcrA, (mrr-hsdRMS-mcrBC), Phi80lacZ(del)M15, ∆lacX74, deoR, recA1, araD139, (ara-leu)7697, galU, galK, rpsL(SmR), endA1, nupG


 E. coli BL21 (DE3)

E. coli B dcm, ompT, hsdS(rB-mB-), gal



C. thermocellum DSM1313 ∆hpt



M1354 harboring pHS0024

This study


M1354 harboring pHS0024_F97W

This study

  1. The plasmids containing mutagenized genes are presented in Additional file 1: Table S1