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Table 2 Plasmids and strains used in this study

From: Single mutation at a highly conserved region of chloramphenicol acetyltransferase enables isobutyl acetate production directly from cellulose by Clostridium thermocellum at elevated temperatures

Name Descriptions Source
Plasmids
 pNW33N BacillusE. coli shuttle vector, CmR, pBC1 ori for Gram-positive strains, pBR322 ori for E. coli, source of CATSa Bacillus Genetic Stock Center
 pETDuet-1 pBR322 ori, AmpR, lacI, T7lac promoter Novagen
 pET_CATSa CATSa wild-type encoding gene between BamHI, SacI site, pETDuet-1 backbone, 6× His-tag at N-terminus This study
 pET_CATSa F97W F97W site-directed variant, pET_CATSa backbone This study
 pHS0024 CATSa wild-type gene under C. thermocellum PgapDH promoter, downstream of Clo1313_2927 for the transcription terminator, tdk operon under cbp promoter substituting with the native cat selection marker, pNW33N plasmid backbone This study
 pHS0024_F97W CATSa F97W site-directed mutated from pHS0024 This study
Strains
 E. coli Top10 Host for molecular cloning, mcrA, (mrr-hsdRMS-mcrBC), Phi80lacZ(del)M15, ∆lacX74, deoR, recA1, araD139, (ara-leu)7697, galU, galK, rpsL(SmR), endA1, nupG Invitrogen
 E. coli BL21 (DE3) E. coli B dcm, ompT, hsdS(rB-mB-), gal Invitrogen
 M1354 C. thermocellum DSM1313 ∆hpt [37]
 HSCT0101 M1354 harboring pHS0024 This study
 HSCT0102 M1354 harboring pHS0024_F97W This study
  1. The plasmids containing mutagenized genes are presented in Additional file 1: Table S1