Table 3 Bacterial strains and plasmids used in this study
Strains/plasmids | Relevant characteristics | Source/references |
|---|---|---|
Bacterial strains | ||
 E. coli | ||
 DH5α | Host cells for gene cloning and plasmids amplification | Invitrogen |
 DH10B | Strain used to methylate the vector | Invitrogen |
C. acetobutylicum | ||
 ATCC 824 | Wild-type strain | ATCC |
 L7 | Adapted from C. acetobutylicum ATCC 824 | [21] |
 L7(pPthl) | Control strain, L7 containing the control plasmid pIMP1-Pthl | This study |
 L7(GlcG) | L7 containing the plasmid pIMP1-Pthl-glcG | This study |
 L7(ΔGlcG) | PTSGlcG-deficient strain via intron-mediated knockout | This study |
Plasmids | ||
 pAN1 | Φ3TI, p15A origin; Sper | [39] |
 PSY6 | Group II intron, ltrA | [41] |
 pIMP1 | Ampr; MLSr; repL, ColE1 origin, shuttle vector | [11] |
 pIMP1-Pthl | Control vector carrying thl promoter, derived from pIMP1 | [11] |
 pIMP1-Pthl-glcG | Derived from pIMP1-Pthl with glcG overexpression in L7 | This study |
 PSY-GlcG | Vector for intron insertion at 269/270 bp of glcG in L7 | This study |