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Table 3 Bacterial strains and plasmids used in this study

From: Pleiotropic regulation of a glucose-specific PTS in Clostridium acetobutylicum for high-efficient butanol production from corn stover without detoxification

Strains/plasmidsRelevant characteristicsSource/references
Bacterial strains
 E. coli
 DH5αHost cells for gene cloning and plasmids amplificationInvitrogen
 DH10BStrain used to methylate the vectorInvitrogen
C. acetobutylicum
 ATCC 824Wild-type strainATCC
 L7Adapted from C. acetobutylicum ATCC 824[21]
 L7(pPthl)Control strain, L7 containing the control plasmid pIMP1-PthlThis study
 L7(GlcG)L7 containing the plasmid pIMP1-Pthl-glcGThis study
 L7(ΔGlcG)PTSGlcG-deficient strain via intron-mediated knockoutThis study
Plasmids
 pAN1Φ3TI, p15A origin; Sper[39]
 PSY6Group II intron, ltrA[41]
 pIMP1Ampr; MLSr; repL, ColE1 origin, shuttle vector[11]
 pIMP1-PthlControl vector carrying thl promoter, derived from pIMP1[11]
 pIMP1-Pthl-glcGDerived from pIMP1-Pthl with glcG overexpression in L7This study
 PSY-GlcGVector for intron insertion at 269/270 bp of glcG in L7This study