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Fig. 1 | Biotechnology for Biofuels

Fig. 1

From: Integrated strain- and process design enable production of 220 g L−1 itaconic acid with Ustilago maydis

Fig. 1

Itaconate production and growth of engineered U. maydis strains. Itaconate concentration (a), growth (OD600, b), pH (c) and DIC images at an magnification of 630× (di) with ∆cyp3 ∆Pria1::Petef #1 (green, filled inverted triangle) and ∆cyp3 ∆Pria1::Petef #2 (black, filled circle) in comparison to ∆cyp3 Petefria1 (red, filled diamond) during shake flask cultivation in screening medium with 30 mM MES (open symbols) or 33 g L−1 CaCO3 (filled symbols) with 50 g L−1 glucose. Error bars indicate the standard error of the mean (n = 3)

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