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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: Integrated strain- and process design enable production of 220 g L−1 itaconic acid with Ustilago maydis

Fig. 2

Itaconate production and growth of morphology-engineered U. maydis strains. U. maydiscyp3 ∆Pria1::Petef (black, da1–6, b1–3) and U. maydiscyp3 ∆Pria1::Peteffuz7 (blue, dc1–6, d1–3). Itaconate concentration (a), pH course (b), DIC images at a magnification of 630 (c) and a 24 well plate during System Duetz® cultivation in screening medium with 30 mM MES (open circle, da1–3+c1–3), 100 mM MES (circle with upper half filled, da4–6+c4–6) and 33 g L−1 CaCO3 (filled circle, db1–3+d1–3) and 50 g L−1 glucose. Error bars indicate the standard error of the mean (n = 3)

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