Fig. 2

Itaconate production and growth of morphology-engineered U. maydis strains. U. maydis ∆cyp3 ∆P_ria1::P_etef (black, d_{a1–6, b1–3}) and U. maydis ∆cyp3 ∆P_ria1::P_etef ∆fuz7 (blue, d_{c1–6, d1–3}). Itaconate concentration (a), pH course (b), DIC images at a magnification of 630 (c) and a 24 well plate during System Duetz^® cultivation in screening medium with 30 mM MES (open circle, d_{a1–3+c1–3}), 100 mM MES (circle with upper half filled, d_{a4–6+c4–6}) and 33 g L⁻¹ CaCO₃ (filled circle, d_{b1–3+d1–3}) and 50 g L⁻¹ glucose. Error bars indicate the standard error of the mean (n = 3)