Fig. 3
Comparison of aqueous and total itaconate concentrations in cultures of engineered U. maydis strains. Itaconate production (filled circle), glucose consumption (filled square) and macroscopic image of a 24-well plate after 96 h (f) of U. maydis ∆cyp3 Petefria1 (a, f(a1–3)), ∆cyp3 ∆Pria1::Petef (b, f(a4–6)), ∆cyp3 ∆Pria1::Petef ∆fuz7 (c, f(b1–3)) and ∆cyp3 ∆Pria1::Petef ∆fuz7 PetefmttA (d, f(b4–6)) during System Duetz® cultivation in screening medium with 66 g L−1 CaCO3 and 100 g L−1 glucose. Dotted lines represent samples treated with HCl and continuous lines represents untreated supernatant samples. Error bars indicate the standard error of the mean (n = 3). Glucose values were combined for untreated and treated samples (n = 6)