Fig. 4
From: Elimination of the flavodiiron electron sink facilitates long-term H2 photoproduction in green algae
Long-term H2 photoproduction under the 1-s light/9-s dark pulse illumination protocol in C. reinhardtii CC-4533 and in the flv 208 mutant. The cells were grown in TP medium for 2 days in 50 µmol photons m−2 s−1 bubbling with 3% CO2 and were shifted to 1/9 pulse illumination without further adjustment (except 20-min purging with Ar). a Specific H2 production yield during 5 days of the experiment. b Photochemical efficiency of PSII (FV/FM) monitored during 5 days of H2 production under the 1/9 pulse illumination protocol. Values for a and b are mean of 6 independent replicates (± SD). c In vitro hydrogenase activity during 3 days of H2 production. Values are mean of 3 independent replicates (± SD). b, c No statistical significance between wt CC-4533 and flv 208. c Statistical significance levels between each time interval (5–24, 24–48 h and 48–72 h): *p < 0.05; **p < 0.01; ***p < 0.001. d Western blot analysis for the semiquantitative determination of HYDA1/A2 content, the first three lanes (200, 100, 50% of 0 h CC-4533) are for approximate quantitation of the proteins