Use of the lignocellulose-degrading bacterium Caldicellulosiruptor bescii to assess recalcitrance and conversion of wild-type and transgenic poplar

Table 2 Selected poplar wood properties with C. bescii treatment data

Line	Target genes	Transcript^b (% of Ctrl)	Lignin^b (wt%)	Total carb^b (wt%)	Height^b (% of Ctrl)	Diameter^b (% of Ctrl)	Stem volume^b (% of Ctrl)	C. bescii solubilization (wt%)	C. bescii fermentation products (mM)
Wild type	–	–	21.7	69.9	100%	100%	100%	20.1 (0.37)	7.3 (0.15)
i20-5	C3H3	13	9.9	86.6	62.2	94.6	49.9	79.3 (0.80)	29.6 (0.48)
i20-10	C3H3	17	13.3	80.7	90.8	109.0	94.7	52.0 (0.71)	22.5 (0.18)
i69-4	C3H3	14	11.9	96.5	66.7	101.8	60.8	64.4 (0.38)	28.1 (0.38)
	C4H1	9
	C4H2	27
i69-13	C3H3	20	13.6	91.1	71.5	103.6	67.4	51.1 (0.83)	23.3 (0.55)
	C4H1	14
	C4H2	36
a10-8	C4H1	23	20.4	66.4	105.7	101.1	55.8	15.9 (0.43)	6.8 (0.15)
a12-10^a	4CL3	16	17.4	70.1	ND	ND	ND	21.5 (0.74)	11.7 (0.25)
i15-3^a	4CL3	3	19.1	71.6	ND	ND	ND	28.5 (0.21)	11.8 (0.06)
i15-3^a	4CL5	10	19.1	71.6	ND	ND	ND	28.5 (0.21)	11.8 (0.06)
i33-5	CAD1	5	21.9	65.6	85.1	103.6	81.1	15.0 (0.19)	8.5 (0.19)
i33-5	CAD1	94	21.4	72.0	96.4	109.0	100.8	24.2 (0.62)	9.1 (0.03)
i35-7	CAD1	6	13.8	68.9	46.6	77.5	26.2	78.2 (0.68)	27.4 (0.17)
i35-7	CAD2	81	13.8	68.9	46.6	77.5	26.2	78.2 (0.68)	27.4 (0.17)
i24-1	CCoAOMT1	6	19.7	72.0	69.5	75.2	47.9	21.5 (0.96)	8.9 (0.10)
i24-1	CCoAOMT2	7	19.7	72.0	69.5	75.2	47.9	21.5 (0.96)	8.9 (0.10)
i21-6^a	CCoAOMT3	24	18.7	68.9	94.2	95.7	99.7	26.6 (1.1)	9.9 (0.02)
i30-1	AldOMT2	5	16.4	71.4	65.5	82.8	52.9	46.7 (0.38)	18.8 (0.14)
i6-9	PAL1	4	18.4	74.3	78.3	76.6	66.1	30.8 (0.53)	12.7 (0.09)
i6-9	PAL2	4	18.4	74.3	78.3	76.6	66.1	30.8 (0.53)	12.7 (0.09)
i8-1	PAL1	31	17.9	70.0	83.7	107.5	126.0	28.5 (1.4)	11.3 (0.29)
	PAL2	62
	PAL3	38
	PAL4&5	120
a4-3	PAL5	21	14.5	74.9	82.2	83.6	80.7	55.7 (0.47)	9.1 (0.11)
i19-4^a	HCT1	81	17.6	76.7	47.9	60.7	23.9	41.2 (0.08)	15.8 (0.19)
i19-4^a	HCT6	44	17.6	76.7	47.9	60.7	23.9	41.2 (0.08)	15.8 (0.19)

Note that transgenic poplar lines with more than one target gene (and accompanying transcript level) are listed in subsequent rows below the first target gene
Standard deviation for n = 3 technical replicate fermentations shown in parenthesis for solubilization and fermentation products
Further data on poplar samples and properties including carbohydrate composition, lignin properties, and enzymatic saccharification data is available in supplementary sections of [28]
^aBiomass samples in which technical replicate biomass samples were combined to obtain sufficient material quantities for this study. Values for biomass samples were averaged. Refer to Additional file 1: Table S1 for data on each biomass sample and calculation of average
^bPreviously published data from [28]

ISSN: 2731-3654