In silico evolution of Aspergillus niger organic acid production suggests strategies for switching acid output

Table 6 Fluxes used to calculate estimates of target acid yield and time of substrate depletion for fitness evaluation

Growth phase 1 t₀ < t < t_c	\(\mu_{1}\)	Specific growth rate
	\(f_{1}\)	Substrate input flux (e.g. glucose)
	\(q\)	External phosphate input flux
Growth phase 2 t_c < t < t_f	\(\mu_{2}\)	Specific growth rate
	\(f_{2}\)	Substrate input flux (e.g. glucose)
	\(p_{2}\)	Target acid output flux

The fluxes from the time-point in growth phase 1 were used to estimate \(t_{c}\), \(A_{c}\), and \(S_{c}\), where \(t_{c}\) is the time of external phosphate depletion and switch to growth phase 2, and \(A_{c}\) and \(S_{c}\) are the amounts of biomass and substrate at \(t_{c}\), respectively

ISSN: 2731-3654