Table 4 Primers used in this study
Primer name | Primer use | Primer sequence (5′→3′) |
|---|---|---|
VP20 | GapB promotor, PstI | ATCACTGCAGGAATAAAAATTACTGACAGC |
VP19 | GapB promotor, SalI | TATCAGTCGACTAGTAGTTTCCTCCTTATAG |
P001 | ddrAB + gapB, ups., PstI | ACGCCTGCAGGAATAAAAATTACTGACAGCC |
P002 | ddrAB, dwn., SalI | TGCGGTCGACTTATTCATCTTGTTGTTCACC |
P038 | sadB + gapB, ups., gibson | CCCTATAAGGAGGAAACTACTAATGAAAGCATTAGTATATCATGGAG |
P039 | sadB, dwn., gibson | AATTCTGTGTTGCGCATGCGGGTACCTTATGCTGCTCCT |
P041 | pJM001, gibson | TCGAGCTCCATGGCATATG |
P036 | pJM001, gibson | TAGTAGTTTCCTCCTTATAGGGATTAGTTAATTAAATACCATACCACCATCA |