Gene targets for engineering osmotolerance in Caldicellulosiruptor bescii

Table 7 Genes found to have relatively low normalized expression values in strain ORCB002

Locus tag	Annotation	log₂(ORCB002/JWCB005)			Average normalized read counts
Locus tag	Annotation	Glycerol	Glucose	NaCl	ORCB002	JWCB005	ORCB001
B5X54_RS04240	DNA-binding protein	− 6.6	− 6.4	− 6.8	0.58	366	346
B5X54_RS04275	Transcriptional regulator	− 6.5	− 7.5	− 7.8	0.57	659	773
B5X54_RS04280	DNA primase	− 6.9	− 7.5	− 8.3	0.57	824	968
B5X54_RS04285	Hypothetical protein	− 7.1	− 7.5	− 7.7	0.57	856	828
B5X54_RS04290	Site-specific integrase	− 6.6	− 5.6	− 6.2	0.12	300	277
B5X54_RS04295	tRNA-Pro	− 5.6	− 5.2	− 5.7	1.6	212	220
B5X54_RS07485	Dihydroorotate dehydrogenase	− 6.6	− 6.9	− 6.8	0.59	358	383
B5X54_RS07490	Histidine phosphatase family protein	− 6.5	− 6.6	− 7.0	0.62	350	401
B5X54_RS07495	Orotate phosphoribosyltransferase	− 6.8	− 6.9	− 6.9	1	467	523
B5X54_RS07500	Hypothetical protein	− 6.6	− 6.4	− 6.6	0.39	290	304

Many of these very low expression values are artefactual, and instead the result of genomic alterations in this strain (as confirmed by genome resequencing of the ORCB002 strain displayed in Table 2). All differential expression log₂ values are statistically significant (Wald test, Benjamini–Hochberg adjusted p value < 0.05, n = 3)

ISSN: 2731-3654