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Table 1 Parameters that were evaluated and the resulting hydrolysis yields for fed-batch operations at high-solids loading

From: Constraints and advances in high-solids enzymatic hydrolysis of lignocellulosic biomass: a critical review

BiomassPretreatmentEnzymeEnzyme dosageFeeding strategyTime of feeding (h)Final solids loading (%)Enzyme addition modeTime (h)Glucose yield (%)References
Sugarcane bagasseLow-temperature aqueous ammonia soakedCellic® CTec210 FPU/g DM12% + 8% + 5% + 5% + 5% + 5%3, 8, 12, 18, 2440Whole96~ 62[128]
Sugarcane bagasseAlkaliCellic® CTec34 FPU/g DM10% + 5% + 4% + 3%8, 12, 1622Whole7276[127]
Sugarcane bagasseAlkali organosolvCellic® CTec23 FPU/g substrate8% + 4% + 4% + 4%6, 12, 1820Whole7251[118]
Sugarcane bagasseAlkaliCellic® CTec210 FPU/g substrate12% + 7% + 7% + 7%6, 12, 2433Whole120~ 60[144]
Sugarcane bagasseFormilineCellulase from Novozymees10 FPU/g DM6.66% + 6.66% + 6.66%12, 3620Whole21080[146]
Corn stoverSteam explosionCellic® CTec210 FPU/g glucan15% + 7.5% + 7.5%12, 2430Whole12060[156]
Corn stoverDilute acidCellic® CTec2 + Cellic® HTec25 FPU/g substrate9% + 3% + 3%12, 2415Whole7276[145]
Corn stoverSteam explosion and NaOH–H2O2Cellulase from Sunson20 FPU/g TS12% + 6% + 6% + 6%12, 36, 6030Whole144~ 58[72]
Corn stoverDilute acidSpezyme CP10.7 FPU/g celluloseMaintaining 15% of solidsEvery 2425Split28880[159]
Rice strawDilute acidSacchariSEB-C63 FPU/g TS10% + 5% + 5%4, 820Split48~ 77[147]
Rice strawDilute acidCellic® CTec215 FPU/g glucan10% + 10% + 10%12, 2430Whole6076[151]
Sugarcane strawHydrothermalCellic® CTec210 FPU/g substrate2.5% + 2.5% + 2.5% + 2.5% + 2.5% + 2.5% + 2.5% + 2.5% + 2.5% + 2.5% + 2.5% + 2.5%1, 2, 4, 8, 12, 18, 24, 30, 36, 42, 4830Split72~ 60[148]
CorncobDilute acid with alkaliCellulase from Sino Biotechnology Co.10 FPU/g DM5% + 5% + 5% + 5%6, 12, 1820Split7259[104]
Brewers spent grainsDilute acid hydrothermalCellic® CTec210 FPU/g biomass12.5% + 12.5%2425Whole48~ 35[155]
Barley strawSteamCelluclast® + Novozyme® 1887.5 FPU/g DM10% + 15%2415Whole7268[154]