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Table 1 Parameters that were evaluated and the resulting hydrolysis yields for fed-batch operations at high-solids loading

From: Constraints and advances in high-solids enzymatic hydrolysis of lignocellulosic biomass: a critical review

Biomass

Pretreatment

Enzyme

Enzyme dosage

Feeding strategy

Time of feeding (h)

Final solids loading (%)

Enzyme addition mode

Time (h)

Glucose yield (%)

References

Sugarcane bagasse

Low-temperature aqueous ammonia soaked

Cellic® CTec2

10 FPU/g DM

12% + 8% + 5% + 5% + 5% + 5%

3, 8, 12, 18, 24

40

Whole

96

~ 62

[128]

Sugarcane bagasse

Alkali

Cellic® CTec3

4 FPU/g DM

10% + 5% + 4% + 3%

8, 12, 16

22

Whole

72

76

[127]

Sugarcane bagasse

Alkali organosolv

Cellic® CTec2

3 FPU/g substrate

8% + 4% + 4% + 4%

6, 12, 18

20

Whole

72

51

[118]

Sugarcane bagasse

Alkali

Cellic® CTec2

10 FPU/g substrate

12% + 7% + 7% + 7%

6, 12, 24

33

Whole

120

~ 60

[144]

Sugarcane bagasse

Formiline

Cellulase from Novozymees

10 FPU/g DM

6.66% + 6.66% + 6.66%

12, 36

20

Whole

210

80

[146]

Corn stover

Steam explosion

Cellic® CTec2

10 FPU/g glucan

15% + 7.5% + 7.5%

12, 24

30

Whole

120

60

[156]

Corn stover

Dilute acid

Cellic® CTec2 + Cellic® HTec2

5 FPU/g substrate

9% + 3% + 3%

12, 24

15

Whole

72

76

[145]

Corn stover

Steam explosion and NaOH–H2O2

Cellulase from Sunson

20 FPU/g TS

12% + 6% + 6% + 6%

12, 36, 60

30

Whole

144

~ 58

[72]

Corn stover

Dilute acid

Spezyme CP

10.7 FPU/g cellulose

Maintaining 15% of solids

Every 24

25

Split

288

80

[159]

Rice straw

Dilute acid

SacchariSEB-C6

3 FPU/g TS

10% + 5% + 5%

4, 8

20

Split

48

~ 77

[147]

Rice straw

Dilute acid

Cellic® CTec2

15 FPU/g glucan

10% + 10% + 10%

12, 24

30

Whole

60

76

[151]

Sugarcane straw

Hydrothermal

Cellic® CTec2

10 FPU/g substrate

2.5% + 2.5% + 2.5% + 2.5% + 2.5% + 2.5% + 2.5% + 2.5% + 2.5% + 2.5% + 2.5% + 2.5%

1, 2, 4, 8, 12, 18, 24, 30, 36, 42, 48

30

Split

72

~ 60

[148]

Corncob

Dilute acid with alkali

Cellulase from Sino Biotechnology Co.

10 FPU/g DM

5% + 5% + 5% + 5%

6, 12, 18

20

Split

72

59

[104]

Brewers spent grains

Dilute acid hydrothermal

Cellic® CTec2

10 FPU/g biomass

12.5% + 12.5%

24

25

Whole

48

~ 35

[155]

Barley straw

Steam

Celluclast® + Novozyme® 188

7.5 FPU/g DM

10% + 15%

24

15

Whole

72

68

[154]