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Table 1 Sequence, translation initiation rate, and ΔGtot of the RBS mutants

From: Biotransformation of keratin waste to amino acids and active peptides based on cell-free catalysis

NameRBS mutant sequence (5′→3′)Translation initiation rate (a.u.)ΔGtotal (kcal/mol)
WTGTAAGAGAGG136,321.8− 4.15
RBS1GAGGGGGAGG1,323,117.0− 15.51
RBS2GAATGGGAGG261,800.7− 11.91
RBS3GAGAAGGAGG1,293,676.2− 15.45
RBS4GAGGAGGAGG1,305,372.8− 15.47
RBS5GTAAGAGAGG136,321.8− 10.45
RBS6GGAGGGGAGG965,565.5− 14.80
RBS7GTAAGAGAGG136,321.8− 10.45
RBS8GGGGGGGAGG1,323,117.0− 15.51
RBS9GGATAGGAGG667,591.9− 13.98
RBS10GGGAGGGAGG866,709.0− 14.56
RBS11GAAAGGTAGG237,121.7− 11.68