Table 1 Sequence, translation initiation rate, and ΔGtot of the RBS mutants
From: Biotransformation of keratin waste to amino acids and active peptides based on cell-free catalysis
Name | RBS mutant sequence (5′→3′) | Translation initiation rate (a.u.) | ΔGtotal (kcal/mol) |
|---|---|---|---|
WT | GTAAGAGAGG | 136,321.8 | − 4.15 |
RBS1 | GAGGGGGAGG | 1,323,117.0 | − 15.51 |
RBS2 | GAATGGGAGG | 261,800.7 | − 11.91 |
RBS3 | GAGAAGGAGG | 1,293,676.2 | − 15.45 |
RBS4 | GAGGAGGAGG | 1,305,372.8 | − 15.47 |
RBS5 | GTAAGAGAGG | 136,321.8 | − 10.45 |
RBS6 | GGAGGGGAGG | 965,565.5 | − 14.80 |
RBS7 | GTAAGAGAGG | 136,321.8 | − 10.45 |
RBS8 | GGGGGGGAGG | 1,323,117.0 | − 15.51 |
RBS9 | GGATAGGAGG | 667,591.9 | − 13.98 |
RBS10 | GGGAGGGAGG | 866,709.0 | − 14.56 |
RBS11 | GAAAGGTAGG | 237,121.7 | − 11.68 |