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Table 1 Sequence, translation initiation rate, and ΔGtot of the RBS mutants

From: Biotransformation of keratin waste to amino acids and active peptides based on cell-free catalysis

Name RBS mutant sequence (5′→3′) Translation initiation rate (a.u.) ΔGtotal (kcal/mol)
WT GTAAGAGAGG 136,321.8 − 4.15
RBS1 GAGGGGGAGG 1,323,117.0 − 15.51
RBS2 GAATGGGAGG 261,800.7 − 11.91
RBS3 GAGAAGGAGG 1,293,676.2 − 15.45
RBS4 GAGGAGGAGG 1,305,372.8 − 15.47
RBS5 GTAAGAGAGG 136,321.8 − 10.45
RBS6 GGAGGGGAGG 965,565.5 − 14.80
RBS7 GTAAGAGAGG 136,321.8 − 10.45
RBS8 GGGGGGGAGG 1,323,117.0 − 15.51
RBS9 GGATAGGAGG 667,591.9 − 13.98
RBS10 GGGAGGGAGG 866,709.0 − 14.56
RBS11 GAAAGGTAGG 237,121.7 − 11.68