Fig. 4

Comparison of three ethyl acetate-producing AAT genes under various gene expression levels, induced by 0.01–0.5 mM IPTG or 0.02–1 mM m-toluate. a–c Fermentation product yields of strains expressing the Kma eat1, the Wan eat1 and the Sce atf1, respectively, under the control of the LacI/T7 promoter after 120 h of cultivation. Gene expression was induced with 0.01–0.5 mM IPTG. d–f Fermentation product yields of strains expressing the Kma eat1, the Wan eat1 and the Sce atf1, respectively, under the control of the XylS/Pm promoter after 120 h of cultivation. Strains were grown in sealed and N₂ flushed serum bottles under anaerobic conditions in modified M9 medium at 30 °C and 150 rpm. Genes were expressed in E. coli BW25113 ΔackAΔldhA (DE3) from a series of pET26b plasmids. Succinate and formate were detected but are not shown. Experiments were performed as biological duplicates; error bars represent the standard deviation