Fig. 6

Ethyl acetate production in pH-controlled bioreactors with continuous gas stripping. Two examples of controlled batch fermentations are shown. a, c Fermentation profile of the strain producing Kma trEat1 K-30 in the presence of 0.05 mM IPTG. b, d Fermentation profile of the strain producing Wan trEat1 N-13 in the presence of 0.01 mM IPTG. Strains were grown under anaerobic conditions in minimal medium containing 55 mM glucose. Genes were expressed in E. coli BW25113 ΔackAΔldhA (DE3) from a series of pET26b plasmids. The cumulative mass of ethyl acetate, CO₂ and H₂ removed by gas stripping was divided by the culture volume of the reactor and in case of ethyl acetate added to concentrations measured in the liquid. Experiments were performed as biological duplicates; error bars represent the standard deviation. trEat1 truncated Eat1