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Table 2 Plasmids used in this study

From: Multilevel optimisation of anaerobic ethyl acetate production in engineered Escherichia coli

PlasmidPromoterGene/ProteinSource
pET26bLacI/T7/This study
pET26b:hWanEat1LacI/T7Codon harmonised eat1 from Wickerhamomyces anomalus DSM 6766[17]
pET26b:hKmaEat1LacI/T7Codon harmonised eat1 from Kluyveromyces marxianus DSM 5422[20]
pET26b:opSceAtf1LacI/T7Codon optimised atf1 from Saccharomyces cerevisiae [38]This study
pET26b: XylS/Pm -hWanEat1XylS/PmCodon harmonised eat1 from Wickerhamomyces anomalus DSM 6766This study
pET26b: XylS/Pm hKmaEat1XylS/PmCodon harmonised eat1 from Kluyveromyces marxianus DSM 5422This study
pET26b: XylS/Pm opSceAtf1XylS/PmCodon optimised atf1 from Saccharomyces cerevisiaeThis study
pET26b:hKma trEat1 F-26LacI/T7Kma Eat1 truncated at F-26[20]
pET26b:hKma-trEat1-K30LacI/T7Kma Eat1 truncated at K-30[20]
pET26b:hWan-trEat1-V11LacI/T7Wan Eat1 truncated at V-11[20]
pET26b:hWan-trEat1-N13LacI/T7Wan Eat1 truncated at N-13[20]