Table 2 Plasmids used in this study
From: Multilevel optimisation of anaerobic ethyl acetate production in engineered Escherichia coli
Plasmid | Promoter | Gene/Protein | Source |
|---|---|---|---|
pET26b | LacI/T7 | / | This study |
pET26b:hWanEat1 | LacI/T7 | Codon harmonised eat1 from Wickerhamomyces anomalus DSM 6766 | [17] |
pET26b:hKmaEat1 | LacI/T7 | Codon harmonised eat1 from Kluyveromyces marxianus DSM 5422 | [20] |
pET26b:opSceAtf1 | LacI/T7 | Codon optimised atf1 from Saccharomyces cerevisiae [38] | This study |
pET26b: XylS/Pm -hWanEat1 | XylS/Pm | Codon harmonised eat1 from Wickerhamomyces anomalus DSM 6766 | This study |
pET26b: XylS/Pm hKmaEat1 | XylS/Pm | Codon harmonised eat1 from Kluyveromyces marxianus DSM 5422 | This study |
pET26b: XylS/Pm opSceAtf1 | XylS/Pm | Codon optimised atf1 from Saccharomyces cerevisiae | This study |
pET26b:hKma trEat1 F-26 | LacI/T7 | Kma Eat1 truncated at F-26 | [20] |
pET26b:hKma-trEat1-K30 | LacI/T7 | Kma Eat1 truncated at K-30 | [20] |
pET26b:hWan-trEat1-V11 | LacI/T7 | Wan Eat1 truncated at V-11 | [20] |
pET26b:hWan-trEat1-N13 | LacI/T7 | Wan Eat1 truncated at N-13 | [20] |