Fig. 3
From: Mechanistic insights into pH-dependent H2 photoproduction in bisulfite-treated Chlamydomonas cells
Treatment with optimal pH alleviates the inhibitory effects of NaHSO3 addition on PSII activity (a), cyclic electron transfer around PSI (b), and CO2 assimilation (c–e) in C. reinhardtii cells. a PSII activity was evaluated by calculated Fv/Fm values. b The rate of cyclic electron transfer around PSI was judged by half-time of P700+ dark reduction. c–e Activity of CO2 assimilation was assessed by photosynthetic production of O2 with NaHCO3 as an artificial electron acceptor (c), Rubisco expression levels (d, e). Coomassie Brilliant Blue (CBB) staining profiles of total proteins from untreated cells and NaHSO3-treated cells (including designated time points) at pH 8.0 (d) and pH 7.3 (e) and their immunoblotting using the antibody against RbcL. A 20-µg aliquot of total protein was loaded onto each lane. Values are mean ± SD (n = 5). f Schematic model representing the relationship of H2 photoproduction with its electron source and alternative electron sinks