Acyl-lipid desaturases and Vipp1 cooperate in cyanobacteria to produce novel omega-3 PUFA-containing glycolipids

Poole, Leslie B.; Parsonage, Derek; Sergeant, Susan; Miller, Leslie R.; Lee, Jingyun; Furdui, Cristina M.; Chilton, Floyd H.

doi:10.1186/s13068-020-01719-7

Biotechnology for Biofuels and Bioproducts

Table 2 Lipidomics analysis of molecular species in wild type (WT) and engineered (+pDBV) Leptolyngbya BL0902

From: Acyl-lipid desaturases and Vipp1 cooperate in cyanobacteria to produce novel omega-3 PUFA-containing glycolipids

Molecular species^a	MGDG		DGDG		SQDG		PG
Molecular species^a	WT	+pDBV	WT	+pDBV	WT	+pDBV	WT	+pDBV
14:0/16:0			0.005 ± 0.001	0.002 ± 0.001			0.001 ± 0.001	0.000 ± 0.000
14:0/18:3			0.013 ± 0.001	0.010 ± 0.003
14:0/18:4	0.000 ± 0.000	0.009 ± 0.002	0.000 ± 0.000	0.006 ± 0.002
16:0/16:0	0.066 ± 0.024	0.129 ± 0.038	0.043 ± 0.022	0.150 ± 0.111	0.008 ± 0.005	0.016 ± 0.015	0.037 ± 0.023	0.113 ± 0.037
16:0/16:1	0.161 ± 0.028	0.201 ± 0.080	0.045 ± 0.018	0.078 ± 0.024			0.077 ± 0.025	0.089 ± 0.028
16:0/16:2	0.021 ± 0.006	0.016 ± 0.005	0.011 ± 0.004	0.006 ± 0.001			0.004 ± 0.002	0.007 ± 0.003
16:0/16:3	0.001 ± 0.000	0.040 ± 0.018
16:0/16:4	0.000 ± 0.000	0.010 ± 0.003
16:0/17:0	0.002 ± 0.001	0.007 ± 0.002	0.002 ± 0.002	0.012 ± 0.005			0.000 ± 0.001	0.001 ± 0.002
16:0/17:1	0.040 ± 0.010	0.066 ± 0.020	0.009 ± 0.004	0.016 ± 0.003			0.013 ± 0.005	0.044 ± 0.020
16:0/17:2	0.028 ± 0.006	0.034 ± 0.012	0.011 ± 0.003	0.009 ± 0.002			0.005 ± 0.002	0.001 ± 0.002
16:0/17:3	0.020 ± 0.008	0.024 ± 0.004	0.010 ± 0.006	0.010 ± 0.006			0.000 ± 0.000	0.014 ± 0.005
16:0/17:4	0.000 ± 0.000	0.045 ± 0.014	0.000 ± 0.000	0.015 ± 0.003
16:0/18:0	0.008 ± 0.005	0.020 ± 0.019	0.007 ± 0.008	0.029 ± 0.011			0.002 ± 0.002	0.006 ± 0.002
16:0/18:1	0.410 ± 0.088	0.488 ± 0.180	0.087 ± 0.044	0.185 ± 0.057			0.275 ± 0.068	0.397 ± 0.176
16:0/18:2	0.908 ± 0.179	0.501 ± 0.132	0.311 ± 0.059	0.145 ± 0.039			0.484 ± 0.177	0.093 ± 0.022
16:0/18:3	1.166 ± 0.421	0.469 ± 0.254	0.491 ± 0.160	0.424 ± 0.118			0.027 ± 0.013	1.325 ± 0.636
16:0/18:4	0.090 ± 0.011	2.507 ± 0.665	0.022 ± 0.013	1.462 ± 0.321			0.001 ± 0.001	0.046 ± 0.021
16:0/19:1	0.007 ± 0.001	0.017 ± 0.006	0.002 ± 0.001	0.004 ± 0.001
16:0/19:2	0.006 ± 0.004	0.002 ± 0.001	0.003 ± 0.002	0.001 ± 0.000
16:0/19:3	0.002 ± 0.001	0.007 ± 0.001
16:0/20:0	0.000 ± 0.000	0.001 ± 0.001
16:0/20:2	0.004 ± 0.003	0.003 ± 0.001	0.003 ± 0.003	0.001 ± 0.000
16:0/20:3	0.012 ± 0.009	0.033 ± 0.010	0.005 ± 0.004	0.010 ± 0.003			0.001 ± 0.002	0.004 ± 0.001
16:0/20:4	0.006 ± 0.004	0.174 ± 0.065					0.000 ± 0.000	0.037 ± 0.021
16:0/20:5	0.077 ± 0.010	0.122 ± 0.070
16:1/16:1	0.005 ± 0.001	0.007 ± 0.002	0.005 ± 0.001	0.003 ± 0.001
16:1/16:2	0.001 ± 0.001	0.000 ± 0.000
16:1/17:3	0.001 ± 0.001	0.001 ± 0.000	0.001 ± 0.001	0.000 ± 0.000
16:1/18:2	0.022 ± 0.004	0.171 ± 0.036	0.019 ± 0.005	0.004 ± 0.001
16:1/18:3	0.080 ± 0.028	0.018 ± 0.011	0.076 ± 0.030	0.026 ± 0.009			0.000 ± 0.000	0.006 ± 0.001
16:1/18:4	0.003 ± 0.001	0.166 ± 0.051	0.002 ± 0.001	0.115 ± 0.027
17:0/18:3	0.001 ± 0.000	0.002 ± 0.001
17:0/18:4	0.001 ± 0.000	0.006 ± 0.004
17:1/18:3	0.001 ± 0.001	0.001 ± 0.001	0.001 ± 0.001	0.000 ± 0.000
17:5/23:2					1.089 ± 0.123	2.172 ± 0.449
17:5/23:3					0.005 ± 0.003	0.006 ± 0.003
18:0/18:1	0.001 ± 0.001	0.002 ± 0.001					0.001 ± 0.000	0.002 ± 0.001
18:1/18:2	0.005 ± 0.005	0.001 ± 0.001					0.001 ± 0.000	0.008 ± 0.003
18:1/18:3			0.002 ± 0.001	0.071 ± 0.027
18:2/18:2	0.009 ± 0.003	0.011 ± 0.004	0.003 ± 0.002	0.001 ± 0.001
18:2/18:3	0.005 ± 0.004	0.005 ± 0.004	0.005 ± 0.003	0.006 ± 0.005
18:3/18:3	0.003 ± 0.003	0.001 ± 0.001	0.002 ± 0.001	0.000 ± 0.000
18:3/18:4	0.000 ± 0.000	0.032 ± 0.015	0.000 ± 0.000	0.012 ± 0.004
18:4/18:4	0.000 ± 0.000	0.049 ± 0.037
18:4/20:4	0.000 ± 0.000	0.012 ± 0.009

Values reported are estimates of mg/g of total fatty acid based on (i) normalized peak areas from LC/MS, (ii) fraction of total peak area for each species in a sample, and (iii) known total fatty acid yield for that organism from GC–FID analysis. This treatment assumes that all species exhibit the same ionization efficiency. Shown are mean ± standard deviation for wild-type (WT) Leptolyngbya BL0902 and the same strain with pDBV (n = 4 for each). Molecular species containing at least one acyl group with SDA (18:4), ETA (20:4) or EPA (20:5) are underlined. No entry means that the species was not observed in the WT or pDBV samples in that category
MGDG monogalactosyldiacylglycerol, DGDG digalactosyldiacylglycerol, SQDG sulfoquinovosyldiacylglycerol, PG phosphatidylglycerol
^aMolecular species shown are for the two fatty acyl chains, giving carbon chain length and number of double bonds for each (e.g., 16:0 means 16 carbons long with 0 double bonds)

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