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Table 2 Lipidomics analysis of molecular species in wild type (WT) and engineered (+pDBV) Leptolyngbya BL0902

From: Acyl-lipid desaturases and Vipp1 cooperate in cyanobacteria to produce novel omega-3 PUFA-containing glycolipids

Molecular speciesaMGDGDGDGSQDGPG
WT+pDBVWT+pDBVWT+pDBVWT+pDBV
14:0/16:0  0.005 ± 0.0010.002 ± 0.001  0.001 ± 0.0010.000 ± 0.000
14:0/18:3  0.013 ± 0.0010.010 ± 0.003    
14:0/18:40.000 ± 0.0000.009 ± 0.0020.000 ± 0.0000.006 ± 0.002    
16:0/16:00.066 ± 0.0240.129 ± 0.0380.043 ± 0.0220.150 ± 0.1110.008 ± 0.0050.016 ± 0.0150.037 ± 0.0230.113 ± 0.037
16:0/16:10.161 ± 0.0280.201 ± 0.0800.045 ± 0.0180.078 ± 0.024  0.077 ± 0.0250.089 ± 0.028
16:0/16:20.021 ± 0.0060.016 ± 0.0050.011 ± 0.0040.006 ± 0.001  0.004 ± 0.0020.007 ± 0.003
16:0/16:30.001 ± 0.0000.040 ± 0.018      
16:0/16:40.000 ± 0.0000.010 ± 0.003      
16:0/17:00.002 ± 0.0010.007 ± 0.0020.002 ± 0.0020.012 ± 0.005  0.000 ± 0.0010.001 ± 0.002
16:0/17:10.040 ± 0.0100.066 ± 0.0200.009 ± 0.0040.016 ± 0.003  0.013 ± 0.0050.044 ± 0.020
16:0/17:20.028 ± 0.0060.034 ± 0.0120.011 ± 0.0030.009 ± 0.002  0.005 ± 0.0020.001 ± 0.002
16:0/17:30.020 ± 0.0080.024 ± 0.0040.010 ± 0.0060.010 ± 0.006  0.000 ± 0.0000.014 ± 0.005
16:0/17:40.000 ± 0.0000.045 ± 0.0140.000 ± 0.0000.015 ± 0.003    
16:0/18:00.008 ± 0.0050.020 ± 0.0190.007 ± 0.0080.029 ± 0.011  0.002 ± 0.0020.006 ± 0.002
16:0/18:10.410 ± 0.0880.488 ± 0.1800.087 ± 0.0440.185 ± 0.057  0.275 ± 0.0680.397 ± 0.176
16:0/18:20.908 ± 0.1790.501 ± 0.1320.311 ± 0.0590.145 ± 0.039  0.484 ± 0.1770.093 ± 0.022
16:0/18:31.166 ± 0.4210.469 ± 0.2540.491 ± 0.1600.424 ± 0.118  0.027 ± 0.0131.325 ± 0.636
16:0/18:40.090 ± 0.0112.507 ± 0.6650.022 ± 0.0131.462 ± 0.321  0.001 ± 0.0010.046 ± 0.021
16:0/19:10.007 ± 0.0010.017 ± 0.0060.002 ± 0.0010.004 ± 0.001    
16:0/19:20.006 ± 0.0040.002 ± 0.0010.003 ± 0.0020.001 ± 0.000    
16:0/19:30.002 ± 0.0010.007 ± 0.001      
16:0/20:00.000 ± 0.0000.001 ± 0.001      
16:0/20:20.004 ± 0.0030.003 ± 0.0010.003 ± 0.0030.001 ± 0.000    
16:0/20:30.012 ± 0.0090.033 ± 0.0100.005 ± 0.0040.010 ± 0.003  0.001 ± 0.0020.004 ± 0.001
16:0/20:40.006 ± 0.0040.174 ± 0.065    0.000 ± 0.0000.037 ± 0.021
16:0/20:50.077 ± 0.0100.122 ± 0.070      
16:1/16:10.005 ± 0.0010.007 ± 0.0020.005 ± 0.0010.003 ± 0.001    
16:1/16:20.001 ± 0.0010.000 ± 0.000      
16:1/17:30.001 ± 0.0010.001 ± 0.0000.001 ± 0.0010.000 ± 0.000    
16:1/18:20.022 ± 0.0040.171 ± 0.0360.019 ± 0.0050.004 ± 0.001    
16:1/18:30.080 ± 0.0280.018 ± 0.0110.076 ± 0.0300.026 ± 0.009  0.000 ± 0.0000.006 ± 0.001
16:1/18:40.003 ± 0.0010.166 ± 0.0510.002 ± 0.0010.115 ± 0.027    
17:0/18:30.001 ± 0.0000.002 ± 0.001      
17:0/18:40.001 ± 0.0000.006 ± 0.004      
17:1/18:30.001 ± 0.0010.001 ± 0.0010.001 ± 0.0010.000 ± 0.000    
17:5/23:2    1.089 ± 0.1232.172 ± 0.449  
17:5/23:3    0.005 ± 0.0030.006 ± 0.003  
18:0/18:10.001 ± 0.0010.002 ± 0.001    0.001 ± 0.0000.002 ± 0.001
18:1/18:20.005 ± 0.0050.001 ± 0.001    0.001 ± 0.0000.008 ± 0.003
18:1/18:3  0.002 ± 0.0010.071 ± 0.027    
18:2/18:20.009 ± 0.0030.011 ± 0.0040.003 ± 0.0020.001 ± 0.001    
18:2/18:30.005 ± 0.0040.005 ± 0.0040.005 ± 0.0030.006 ± 0.005    
18:3/18:30.003 ± 0.0030.001 ± 0.0010.002 ± 0.0010.000 ± 0.000    
18:3/18:40.000 ± 0.0000.032 ± 0.0150.000 ± 0.0000.012 ± 0.004    
18:4/18:40.000 ± 0.0000.049 ± 0.037      
18:4/20:40.000 ± 0.0000.012 ± 0.009      
  1. Values reported are estimates of mg/g of total fatty acid based on (i) normalized peak areas from LC/MS, (ii) fraction of total peak area for each species in a sample, and (iii) known total fatty acid yield for that organism from GC–FID analysis. This treatment assumes that all species exhibit the same ionization efficiency. Shown are mean ± standard deviation for wild-type (WT) Leptolyngbya BL0902 and the same strain with pDBV (n = 4 for each). Molecular species containing at least one acyl group with SDA (18:4), ETA (20:4) or EPA (20:5) are underlined. No entry means that the species was not observed in the WT or pDBV samples in that category
  2. MGDG monogalactosyldiacylglycerol, DGDG digalactosyldiacylglycerol, SQDG sulfoquinovosyldiacylglycerol, PG phosphatidylglycerol
  3. aMolecular species shown are for the two fatty acyl chains, giving carbon chain length and number of double bonds for each (e.g., 16:0 means 16 carbons long with 0 double bonds)