Table 2 Plasmids and strains used in this study
Plasmids/strains | Genotype | Source |
|---|---|---|
E. coli strains | ||
 DH5α | F– endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG purB20 φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17(r –K m +K ), λ– | TransGen Biotech |
 HB101 | supE44, Δ(mcrC-mrr), recA13, ara-14, proA2, lacY1, galK2, rpsL20, xyl-5,mtl-1, leuB6, thi-1 | Takara Bio |
 BL21(DE3) | F– ompT gal dcm lon hsdSB(r –B m –B ) λ(DE3) | TransGen Biotech |
 cscN | BL21(DE3)/pET30a |  |
 ABKm | BL21(DE3)/pACYC184/pET30a | This study |
Plasmids | ||
 pACYC184 | PJ23100-mcr-T7; speR | This study |
 pET30a | cscA, cscB, cscK; kanR | This study |
 pJA | Pcpc560-cscB-TrbcL; speR, kanR | This study |
Cyanobacteria strains | ||
 WT | Wild-type Synechococcus elongatus UTEX 2973 |  |
 cscB+ 2973 | WT/pJA | This study |