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Table 2 Plasmids and strains used in this study

From: Construction and analysis of an artificial consortium based on the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973 to produce the platform chemical 3-hydroxypropionic acid from CO2

Plasmids/strainsGenotypeSource
E. coli strains
 DH5αF endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG purB20 φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17(rKm+K), λTransGen Biotech
 HB101supE44, Δ(mcrC-mrr), recA13, ara-14, proA2, lacY1, galK2, rpsL20, xyl-5,mtl-1, leuB6, thi-1Takara Bio
 BL21(DE3)F ompT gal dcm lon hsdSB(rBmB) λ(DE3)TransGen Biotech
 cscNBL21(DE3)/pET30a 
 ABKmBL21(DE3)/pACYC184/pET30aThis study
Plasmids
 pACYC184PJ23100-mcr-T7; speRThis study
 pET30acscA, cscB, cscK; kanRThis study
 pJAPcpc560-cscB-TrbcL; speR, kanRThis study
Cyanobacteria strains
 WTWild-type Synechococcus elongatus UTEX 2973 
 cscB+ 2973WT/pJAThis study