Fig. 6

Schematic representation of the Clostridium saccharoperbutylacetonicum N1-4 (HMT) genomic region comprising genes (from Cspa_c45260/hag3 to Cspa_c44960/hag2) of the flagellar regulon; flgC and fliA genes are shown in red (a) and transcriptional analyses of fliA (b) and flgC (c) using qRT-PCR in N1-4 (HMT) and various QSS mutants. RNA samples were extracted from the cell culture after 12 h cultivation in TGY medium. The reported value is mean ± SD. The asterisk indicates that the corresponding gene expression level in that particular strain was significantly different from the WT strain (P < 0.05). Green upwards arrows with tip rightwards (a) indicate promoters of the predicted operons