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Table 1 Strains and plasmids used in this study

From: RRNPP-type quorum-sensing systems regulate solvent formation, sporulation and cell motility in Clostridium saccharoperbutylacetonicum

Strains and plasmidsRelevant characteristicsSource or reference
Strains
 C. saccharoperbutylacetonicum N1-4 (HMT)N1-4, DSM 14923 (=ATCC 27021), WT strainDSMZ
 C. saccharoperbutylacetonicum ΔR1Derived from N1-4, ΔCspa_c00280This study
 C. saccharoperbutylacetonicum ΔR2Derived from N1-4, ΔCspa_c21720This study
 C. saccharoperbutylacetonicum ΔR3Derived from N1-4, ΔCspa_c27220This study
 C. saccharoperbutylacetonicum N1-4-dcas9-R4N1-4 with the expression of pYW-19d-qssR4This study
 C. saccharoperbutylacetonicum ΔR5Derived from N1-4, ΔCspa_c56960This study
 C. saccharoperbutylacetonicum ΔR1-R1ΔR1 strain with the expression of pMTL-qssR1This study
 C. saccharoperbutylacetonicum ΔR2-R2ΔR2 strain with the expression of pMTL-qssR2This study
 C. saccharoperbutylacetonicum ΔR3-R3ΔR3 strain with the expression of pMTL-qssR3This study
 C. saccharoperbutylacetonicum N1-4-R4N1-4 strain with the expression of pMTL-qssR4This study
 C. saccharoperbutylacetonicum ΔR5-R5ΔR5 strain with the expression of pMTL-qssR5This study
 C. saccharoperbutylacetonicum N1-4 (pMTL82151)N1-4 strain with the expression of pMTL82151This study
 Clostridium tyrobutyricum ATCC 25755ATCC 25755 (= KCTC 5387)ATCC
 E. coli DH5αF, φ80dlacZΔM1, Δ(lacZYA-argF)U169, deoR, recA1, endA1, hsdR17(rk, m+k), phoA, supE44, λthi-1, gyrA96, relA1NEB
Plasmids
 pYW34CAK ori, Ampr, Ermr, Plac::Cas9, gRNA[24]
 pMTL82151pBP1 ori, catP, ColE1, tra[47]
 pYW19d-BseRICAK ori, Ampr, Ermr, Pthl::dCas9, gRNA[48]
 pYW34-∆qssR1Derived from pYW34, J23119::20-nt gRNA targeting Cspa_c00280, homology armsThis study
 pYW34-∆qssR2Derived from pYW34, J23119::20-nt gRNA targeting Cspa_c21720, homology armsThis study
 pYW34-∆qssR3Derived from pYW34, J23119::20-nt gRNA targeting Cspa_c27220, homology armsThis study
 pYW34-∆qssR5Derived from pYW34, J23119::20-nt gRNA targeting Cspa_c56960, homology armsThis study
 pMTL-qssR1Derived from pMTL82151, with the expression of Cspa_c00280 under the control of cat1 promoter from C. tyrobutyricum ATCC 25755This study
 pMTL-qssR2Derived from pMTL82151, with the expression of Cspa_c21720 under the control of cat1 promoter from C. tyrobutyricum ATCC 25755This study
 pMTL-qssR3Derived from pMTL82151, with the expression of Cspa_c27220 under the control of cat1 promoter from C. tyrobutyricum ATCC 25755This study
 pMTL-qssR4Derived from pMTL82151, with the expression of Cspa_c29260 under the control of cat1 promoter from C. tyrobutyricum ATCC 25755This study
 pMTL-qssR5Derived from pMTL82151, with the expression of Cspa_c56960 under the control of cat1 promoter from C. tyrobutyricum ATCC 25755This study
 pYW19d-qssR4Derived from pYW19d-BseRI, with J23119::20-nt gRNA targeting Cspa_c29260This study