Fig. 2
Characterization of succinate producing strains of S. elongatus. a Growth profiles of strains WT and SA1–SA3 of PCC 7942 and PCC 11801 grown in BG-11 media in under 1% CO2 in a shaker. b Growth profiles of strains S. elongatus PCC 11801 SA4–SA9, c cumulative 120 h titers for strain SA1, SA2, SA3 and wild-type in S. elongatus PCC 11801 and 7942, d cumulative succinate titers at 120 h for strains SA4-SA9 of PCC 11801, e volumetric productivity of succinate of all the S. elongatus PCC 11801 succinate strains for 5 days. f Estimation of fumarate titers in all the S. elongatus PCC 11801 strains. g Estimation of carbon partitioning between succinate and biomass for engineered strains of S. elongatus PCC 11801. The strains were grown in shake flasks, at 1% CO2 in shaker, h estimation of carbon partitioning of S. elongatus PCC 11801 SA3 strain compared under different growth conditions. Diurnal cycle—light and dark condition in multicultivator, different light conditions 200, 400, 600 and 800 µE in multicultivator with 1% CO2 bubbling. Data shown above are mean of three independent experimental sets with error bars indicating standard error of the mean