Fig. 4
Enzymatic assay of key enzymes for the selected engineered strain. Enzymatic assay of a succinate semialdehyde dehydrogenase, SsaD, b PEP Carboxylase, PEPC c citrate synthase, GltA and d isocitrate dehydrogenase, IDH in the representative strains WT, SA2, SA3, SA6 and SA7 was monitored. The cells were harvested in the exponential phase of growth (OD730 ~ 0.8), centrifuged and lysed in a bead beater. Substrate blank was used as a control. Activity is measured in nanomoles/min/mg of crude protein in the lysate. Error bars represent standard error of mean of three trials