Fig. 1

Growth of thermotolerant P. kudriavzevii LC375240 and ethanol production. a Kinetic growth curves of P. kudriavzevii LC375240 in YPD broth were monitored every 0.5 h interval for 20 h using plate reader for the indicated temperatures. b The indicated cell numbers were inoculated on YPD plates and incubated at 30 °C, 37 °C and 42 °C for 24 h. c Ethanol productions from YPD media with 100 g/l, 160 g/l, 200 g/l glucose were determined after 16 h, 40 h and 72 h fermentation at 42 °C. d The indicated cell numbers were inoculated on YPD plates or plates with two pentose sugars as sole carbon sources and incubated at 42 °C for 48 h