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Table 2 List of C4-oxidized XG oligosaccharides identified based on fragmentation patterns in CID-MS/MS present in the NcLPMO9C-TXG-digest

From: Configuration of active site segments in lytic polysaccharide monooxygenases steers oxidative xyloglucan degradation

9C_C4-Ox Peak Nr.

Elution time (min)

m/z ([M−H]−)

MS/MS fragments (m/z)a

Annotation

1

30.7–32.7

1059.4

293 (4), 453 (1), 527 (3), 605 (13), 765 (1), 821 (16), 839 (7), 899 (100), 999 (2), 1041 (14)

O=GGXXX

455 (1), 473 (1), 767 (4), 999 (2), 1041 (14)

O=GXXXG

2

36.4–37.5

1221.5

311 (2), 767 (4), 1001 (5), 1061 (100), 1161 (2), 1203 (34)

O=GGXLX/O=GGXXL

311 (2), 605 (3), 1001 (5), 1061 (100), 1161 (2), 1203 (34)

O=GGLXX

455 (8), 929 (12), 1161 (2), 1203 (39)

O=GX(H4P2)

3

39.7–41.4

1383.7

311 (3), 1141 (1), 1163 (5), 1223 (100), 1365 (7)

O=GG(H5P3)

4

43.0–45.0

1515.5

n.d.

O=GH6P4

  1. Chromatograms, including peak numbers, are shown in Fig. 5. Nomenclature (annotation) according to Fry et al. [15]
  2. aRelative intensities of MS/MS fragments are shown between brackets and informative MS/MS fragments are indicated in italics
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Biotechnology for Biofuels and Bioproducts

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