Fig. 1

In vitro assembly of minicellulosomes on the P. pastoris cell surface. The ultra-high-affinity IM7/CL7 protein pair was used as the dockerin–cohesin pair for the yeast display system. The IM7 proteins were repeatedly displayed for (a) twice or (b) three times on the yeast cell surface. The three cellulases including an endoglucanase (EG), an exoglucanase (CBH) and a β-glucosidase (BGL), as well as a carbohydrate-binding module (CBM) were fused with an N-terminal CL7 tag and recombinantly expressed in E. coli