Fig. 1

Zymogram analysis of the purified CmNAGase. The purified enzyme was analyzed by SDS-PAGE using a 3% stacking gel and a 10% separating gel with addition of SDS. Lane M, protein molecular mass makers; lane 1, purified CmNAGase (20 μg) visualized by Coomassie brilliant blue staining; lane 2, purified CmNAGase staining with 4-MU-GlcNAc and visualized at 340 nm