Fig. 2

Phylogenetic relationships between CmNAGase and other bacterial NAGases from the glycoside hydrolase (GH) family 20. The phylogenetic tree was constructed by the neighbor-joining algorithm based on the amino acid sequence alignment in MEGA 7.0. The amino acid sequence of CmNAGase was aligned with those of the following proteins: GH20 NAGases from Chitiniphilus shinanonensis (WP_018749679), Jeongeupia sp. USM3 (WP_070527884), Aeromonas sp. 10S-24 (BAA92145), Chitinibacter tainanensis (WP_034618561), Pseudoalteromonas piscicida (BAB17855), Shewanella oneidensis (HexB, NP719056), Aeromonas caviae (nagA1, CAH55822), Serratia marcescens (PDB 1QBA), Enterobacter sp. (BAA74506), Vibrio harveyi 650 (Nag2, PDB 6EZR), Aeromonas hydrophila (BAF76001), Bacteroides fragilis NCTC 9343 (PDB 4PYS), V. harveyi (Nag1, ADJ68332), Bacteroides thetaiotaomicron (PDB 6Q63), Paenarthrobacter aurescens TC1 (PDB 3RCN), Arthrobacter sp. (CAB72127), Streptomyces coelicolor (4C7D), Paenibacillus sp. TS12 (Hex2t, AB490156), Paenibacillus sp. TS12 (Hex1t, BAI63641), Bifidobacterium bifidum JCM 1254 (BAI94823), Lactobacillus casei (BAA76352), and Streptococcus gordonii (AAS02154)