Biotechnology for Biofuels and Bioproducts

Table 1 Effect of different metal ions on CmNAGase

From: A novel bacterial β-N-acetyl glucosaminidase from Chitinolyticbacter meiyuanensis possessing transglycosylation and reverse hydrolysis activities

Metal ions	Chemicals	Relative activity (%)
No addition	–	100
EDTA	EDTA	101.4 ± 3.2
Na⁺	NaCl	96.8 ± 6.7
K⁺	KCl	101.9 ± 7.8
Fe²⁺	FeCl₂	97.6 ± 4.2
Ca²⁺	CaCl₂	93.9 ± 6.6
Cu²⁺	CuCl₂	0
Ni²⁺	NiCl₂	106.3 ± 4.9
Zn²⁺	ZnCl₂	0
Mg²⁺	MgCl₂	83.3 ± 6.3
Mn²⁺	MnCl₂	116.3 ± 3.5
Co²⁺	CoCl₂	103.1 ± 3.8
Ba²⁺	BaCl₂	34.7 ± 4.6
Al³⁺	AlCl₃	0
Fe³⁺	FeCl₃	21.3 ± 1.1
Cr³⁺	CrCl₃	4.3 ± 2.5

Samples were preincubated with various mental ions (10 mM) at pH 7.0 (Tris–HCl buffer) and 4 °C for 30 min. The remaining activity was measured with pNP-GlcNAc at pH 7.0 (Tris–HCl buffer) and 40 °C for 10 min. Activity in the absence of any additives was taken as 100%

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