Fig. 3

Generation of PtPTP overexpression strains and molecular analysis of PtPTP expression. a Map of PtPTP overexpression cassettes in pPhat-EF2-PtPTP. b Genomic PCR of the wild-type (WT) and PtPTP-overexpressing strains (PtPTP-OE1, OE2); Upper: PCR for EF2::PTP with primers of EF2-iFw and PTP-iRv (described in A); Middle: PCR for PTP with primers of PTP-iFw and PTP-iRv (described in A); Bottom: PCR for ITS (internal transcribed spacer) ITS, PCR for internal transcribed spacer (1.1 kb); SM, DNA size marker; VC, vector control; c Relative mRNA quantity of PtPTP in the wild-type (WT) and transgenic strains (OE1 and OE2). Error bars represent the mean value from three independent experiments. Statistically significant differences were determined by Student’s t test (***p < 0.001, **p < 0.01, *p < 0.05). d Immunoblotting with anti-PTP for analysis of overexpression of PtPTP in transgenic strains. Band intensities were quantified using Image J software, compared to the wild type