Fig. 1

Assessment of acetic acid tolerance in small-scale fermentations with parent, diploid hybrid and segregant strains. Haploid parental strains MS164 (red, ) and 16D (blue, ), diploid hybrid MS218 (green, ) and a selection of haploid segregants (black). Fermentations were performed at 35 °C, constant stirring at 120 rpm, pH 4.7 in 10 mL (a, b) or 50 mL (c) YPD medium with 40 g/L glucose and supplemented with 8 g/L (a) or 12 g/L (b, c) acetic acid